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inVia: volume images

Raman volume images

You can now determine detailed chemical and property information from volumes within suitable samples.

Which samples can be volume analysed?


  • the presence and size of key biological features, such as the nucleus and organelles within cells
  • inclusion size and shape within gemstones, glasses and other transparent solids
  • the variation in layer thickness of films, such as polymer laminates and multilayer coatings
  • the distribution and size of droplets in emulsions
  • the structure of foams and other delicate materials which cannot be touched
  • complex stress fields which can occur within transparent solids such as diamond

If your sample is transparent to the laser and Raman signal, you can generate volume images.

Raman and volume, an ideal match

Fast acquisition techniques, such as Renishaw's StreamHR™ (with optional EM-CCD capability), can collect large amounts of Raman data rapidly. This capability, coupled with inVia's high confocality, enables you to generate high spatial resolution Raman volume images (voxel data).

Manage refraction at interfaces

Refraction at interfaces can impair the quality of volume images but can be minimised and corrected.

  • Renishaw has tested a wide selection of immersion objectives and cover-slip corrected objectives. These reduce refraction, maintain confocality, and improve performance when looking deep into samples.
  • WiRE software can correct for refractive index differences. You can use this to alter the depth scale so that it reflects true depth within the sample, rather than apparent depth.

Fully understand your sample

You can collect one volume map and understand your material in all three dimensions.

With Renishaw's WiRE software you can process and analyse your 3D data in its entirely, rather than just slice-by-slice. You can apply advanced techniques (such as chemometrics) to the whole volume to extract and process additional information about your sample.

Find out more