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Tissue

A non-invasive and label-free technique, Raman spectroscopy is an ideal analytical tool for tissue analysis.

You can extract a full spectrum of chemical information (from entities such as nucleic acids, proteins and lipids) without the need for targeting biomolecules, markers, stains or dyes. Unlike many other analysis techniques—such as western blot, GC/MS, and MALDI-TOF—Raman analysis does not require the sample to be homogenized.

Accurately and quickly identify tissue layers

Distinguish, identify and demarcate pre-cancerous, cancerous and healthy tissues.

  • No need for conjugation with antibodies: save both money and time when optimising protocols
  • Reliably demarcate and objectively identify anatomical layers in the tissues, based on their total molecular composition. Delineate tumour margins
  • Avoid subjective colourimetry and morphology-based analysis
  • Identify chemical changes in the tissues that have not manifested themselves in morphological changes (e.g. levels of DNA/RNA, glycogen, lipid, protein, lipid phase and DNA integrity)

Understand biological systems

Get a complete chemical analysis of tissues and understand the underlying mechanism of their changes.

Study:

  • the development of an organism
  • the pathogenesis of diseases
  • tissue's response to drugs or stimulants (e.g. chemotherapeutic agents, toxins and anti-inflammatories)

Examine in one procedure:

  • changes in the level and conformation of proteins, lipids, nucleic acids and carbohydrates
  • the presence of mineral deposits (e.g. calcification in breast tissue and atherosclerosis) 
  • the redox states of heme proteins (e.g. neuroglobins and myoglobins)
  • Raman image of tissue layers Raman image of tissue layers

Image tissues

Renishaw's StreamLine™ technology is particularly suited to generating tissue images. Its line-focus geometry enables you to use high laser powers whilst avoiding photothermal damage to the tissue. This maximises signal levels and gives you images in the shortest possible time.

Study uneven surfaces

With StreamLine's Surface capability, you can generate images of samples, even if they are not flat.

Slide scanning automation

You can configure Renishaw's fully automated Raman systems to scan sequentially multiple histological sections and run unattended. This saves you time and enables you to maximise the productivity of your Raman system.

Download an application note

Find out more

You may be interested in these papers:

Kast et al (2014) J Neurooncol doi 10.1007/s11060-014-1536-9
Bonifacio et al (2010) Analyst 135: 3193-3204

Recent news

Raman spectroscopy used to detect radiation damage in cells and tissues during cancer treatment

The Irving K Barber School of Arts and Sciences at the University of British Columbia, Canada, hosts a multidisciplinary group of physics, engineering and radiation oncology scientists. It is interested in detecting and understanding the damage in cells and tissues caused by ionizing radiation used in cancer treatments.

Raman spectroscopy used to study diseases at the Children's Hospital of Michigan

The Children's Hospital of Michigan and Wayne State University use a Renishaw inVia confocal Raman microscope in the study of various diseases, with a major focus on childhood diseases.

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