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Tissue

A non-invasive and label-free technique, Raman spectroscopy is an ideal analytical tool for tissue analysis.

You can extract a full spectrum of chemical information (from entities such as nucleic acids, proteins and lipids) without the need for targeting biomolecules, markers, stains or dyes. Unlike many other analysis techniques—such as western blot, GC/MS, and MALDI-TOF—Raman analysis does not require the sample to be homogenized.

Quickly and accurately identify tissue layers

Distinguish, identify and demarcate pre-cancerous, cancerous and healthy tissues.

  • No need for conjugation with antibodies: save both money and time when optimising protocols
  • Reliably demarcate and objectively identify anatomical layers in the tissues, based on their total molecular composition. Delineate tumour margins
  • Avoid subjective colourimetry and morphology-based analysis
  • Identify chemical changes in the tissues that have not manifested themselves in morphological changes (e.g. levels of DNA/RNA, glycogen, lipid, protein, lipid phase and DNA integrity)

Understand biological systems

Get a complete chemical analysis of tissues and understand the underlying mechanism of their changes.

Study:

  • the development of an organism
  • the pathogenesis of diseases
  • tissue's response to drugs or stimulants (e.g. chemotherapeutic agents, toxins and anti-inflammatories)

Examine in one procedure:

  • changes in the level and conformation of proteins, lipids, nucleic acids and carbohydrates
  • the presence of mineral deposits (e.g. calcification in breast tissue and atherosclerosis)
  • the redox states of heme proteins (e.g. neuroglobins and myoglobins)
  • Raman image of tissue layers Raman image of tissue layers

Image tissues

Renishaw's StreamLine™ technology is particularly suited to generating tissue images. Its line-focus geometry enables you to use high laser powers whilst avoiding photothermal damage to the tissue. This maximises signal levels and gives you images in the shortest possible time.

Study uneven surfaces

With StreamLine's Surface capability, you can generate images of samples, even if they are not flat.

Slide scanning automation

You can configure Renishaw's fully automated Raman systems to scan sequentially multiple histological sections and run unattended. This saves you time and enables you to maximise the productivity of your Raman system.

We're here when you need us

Our specialists have a wealth of experience across a broad range of Raman application areas.

Contact them to find out more about these, or an application that isn't covered here.

Contact our applications team

Downloads: life sciences (tissues)

You may be interested in these papers:

Kast et al (2014) J Neurooncol doi 10.1007/s11060-014-1536-9
Bonifacio et al (2010) Analyst 135: 3193-3204

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